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- PROX1 Antibodies
Proteintech
View all (11) PROX1 antibodies
Cite PROX1 Recombinant Rabbit Monoclonal Antibody (2H12)
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PROX1 Antibody (81778-1-RR) in WB
Various lysates were subjected to SDS PAGE followed by western blot with 81778-1-RR (PROX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
81778-1-RR
Applications
Tested Dilution
Publications
Western Blot(WB)
1:2,000-1:10,000
-
ELISA(ELISA)
Assay-dependent
-
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H12
Immunogen
Recombinant protein
Conjugate
Unconjugated Unconjugated Unconjugated
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: TAEGLSLSLI KSECGDLQDM SEISPYSGSA MQEGLSPNHL KKAKLMFFYT RYPSSNMLKT YFSDVKFNRC ITSQLIKWFS NFREFYYIQM EKYARQAIND GVTSTEELSI TRDCELYRAL NMHYNKANDF EVPERFLEVA QITLREFFNA IIAGKDVDPS WKKAIYKVIC KLDSEVPEIF KSPNCLQELL H
Target Information
The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:Homeobox prospero-like protein PROX1; Prospero homeobox protein 1; prospero-related homeobox 1; PROX-1
UniProt ID:(Human) Q92786
Entrez Gene ID:(Human) 5629
Function(s)
core promoter sequence-specific DNA bindingtranscriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific bindingDNA bindingtranscription factor activity, sequence-specific DNA bindingtranscription factor activity, RNA polymerase II distal enhancer sequence-specific bindingtranscription corepressor activityprotein bindingligand-dependent nuclear receptor bindingtranscription regulatory region DNA bindingDBD domain bindingLBD domain bindinghomeodomain transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promotercell fate determinationkidney developmentliver developmentpositive regulation of endothelial cell proliferationlymphangiogenesislens development in camera-type eyehepatocyte cell migrationtranscription, DNA-templatedbrain developmentcircadian rhythmpositive regulation of cell proliferationnegative regulation of cell proliferationregulation of gene expressionpositive regulation of endothelial cell migrationdorsal spinal cord developmentdentate gyrus developmentcerebellar granule cell differentiationneural tube developmentneuron differentiationskeletal muscle thin filament assemblylung developmentolfactory placode formationpancreas developmentresponse to nutrient levelsregulation of circadian rhythmotic placode formationnegative regulation of sequence-specific DNA binding transcription factor activitynegative regulation of viral genome replicationpositive regulation of cyclin-dependent protein serine/threonine kinase activitypositive regulation of cell cyclenegative regulation of transcription, DNA-templatedpositive regulation of transcription, DNA-templatedpositive regulation of transcription from RNA polymerase II promoteroptic placode formation involved in camera-type eye formationvenous blood vessel morphogenesisventricular cardiac myofibril assemblycardiac muscle cell differentiationatrial cardiac muscle tissue morphogenesisventricular cardiac muscle tissue morphogenesisretina morphogenesis in camera-type eyeembryonic retina morphogenesis in camera-type eyeendocardium formationpositive regulation of sarcomere organizationventricular septum morphogenesisaorta smooth muscle tissue morphogenesispositive regulation of heart growthlymphatic endothelial cell differentiationregulation of transcription involved in lymphatic endothelial cell fate commitmentbranching involved in pancreas morphogenesislens fiber cell morphogenesishepatocyte differentiationnegative regulation of bile acid biosynthetic processhepatocyte proliferationacinar cell differentiationneuronal stem cell population maintenancepositive regulation of cell cycle checkpointpositive regulation of neural precursor cell proliferationpositive regulation of forebrain neuron differentiation
It has to be done as per old AB suggested Products section.
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